Anti-Inflammatory and Antiosteoclastogenic Activities of Parthenolide on Human Periodontal Ligament Cells In Vitro

Figure 5

TRAP staining of osteoclasts cocultured with hPDLCs. (a) TRAP-positive staining in RAW 264.7 cells. TRAP staining of RAW 264.7 cells was performed using assay kit. Images were captured using a Nikon ECLIPSE TS100 microscope with a 10x camera. Representative images are depicted from three patients. (b) Percentage of TRAP-positive MNCs in RAW 264.7 cells. Three randomly selected images were recorded in each treatment group and the number of MNCs was counted. The final data was the average cell number of nine different images from three different patients. Error bars indicate mean ± SEM (). versus the control while versus the LPS group.

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