IKK β -Targeted Anti-Inflammatory Activities of a Butanol Fraction of Artificially Cultivated Cordyceps pruinosa Fruit Bodies

Figure 2

Effect of Cp-BF on production of inflammatory mediators, release of reactive oxygen species, and phagocytic uptake. ((a) and (b)) Levels of NO and TNF-α were determined by Griess assay and ELISA from culture supernatants of RAW264.7 cells treated with Cp-BF and LPS (1 μg/mL) for 24 h. (c) The effect of Cp-BF on phagocytic uptake of RAW264.7 cells was determined by treatment with FITC-dextran (1 mg/mL) for 2 h and the uptake of which was determined by flow cytometric analysis. (d) The effect of Cp-BF on reactive oxygen species (ROS) generation in LPS-treated RAW264.7 cells was determined by incubation with H₂DCFDA (50 μM) and flow cytometric analysis. (e) Cell viability of RAW264.7 and HEK293 cells as determined by MTT assay. ^∗𝑃<0.05 and ^∗∗𝑃<0.01 compared to control.

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