Cytotoxicity of Aporphine, Protoberberine, and Protopine Alkaloids from Dicranostigma leptopodum (Maxim.) Fedde

Table 1

Cytotoxicities of compounds 1–9 from Dicranostigma  leptopodum (Maxim.) Fedde.

Compound	IC50 (𝜇M)^a,b
	H1299	MCF-7	SMMC-7721
Aporphine alkaloids
1	>100	>100	>100
2	>100	>100	70.08 ± 4.63
3	53.58 ± 5.47	72.30 ± 1.72	73.22 ± 2.35
4	>100	>100	>100
5	>100	>100	>100
6	>100	>100	>100
Protoberberine alkaloids
7	28.22 ± 1.03	28.34 ± 2.00	27.77 ± 2.29
Protopine alkaloids
8	>100	>100	>100
9	>100	>100	>100
DOX^c	11.70 ± 1.53	7.82 ± 0.89	2.74 ± 0.34

aIC₅₀ value was the 50% inhibition concentration and calculated from regression lines using five different concentrations in replicate experiments for six times. ^bSolvent used in the cytotoxicity test was DMSO, and purity of compounds under the test is above 90%. ^cDoxorubicin was used as the positive control.