Figure 1: Typical RRLC-QQQ-MS/MS chromatograms of marker chemicals in DBT. (a) The chemical structures of calycosin-7-O-β-D-glucoside, calycosin, ononin, formononetin, astragaloside IV, and ginsenoside Rg1 (internal standard) were shown. (b) The chromatographic method is described in Section 2. The identifications of calycosin-7-O-β-D-glucoside (1), ononin (2), calycosin (3), astragaloside IV (4), formononetin (5), and ginsenoside Rg1 (internal standard, IS) were made by a MS detector. Concentrations of the standards were 0.2 μg/mL, and 0.5 mL of the DBT decoction was used. Representative chromatograms are shown, .