Hydrolysis of Glycosidic Flavonoids during the Preparation of Danggui Buxue Tang: An Outcome of Moderate Boiling of Chinese Herbal Mixture
Figure 5
The herb-induced hydrolysis is in a dose-dependent manner. Under 25°C at pH 5.0, 50 μg of calycosin-7-O-β-D-glucoside, or 50 μg ononin, was included to the water (50 mL) suspended with different amount of ASR or AR powders for 4 h. Both crude and heated (30 min at 100°C) herbal powders were used here. The amounts of calycosin-7-O-β-D-glucoside, calycosin, ononin, and formononetin were determined by RRLC-QQQ-MS/MS. The percentage of hydrolysis rate was determined by the amount of flavonoid or flavonoid glycoside (in molar) determined to that original applied flavonoid glycoside (in molar). (a) Conversion from calycosin-7-O-β-D-glucoside to calycosin. (b) Conversion efficiency of ononin to formononetin. The values are in Mean ± SD, where , each with triplicate samples.
