Ligusticum wallichii Extract Inhibited the Expression of IL-1β after AMI in Rats
Figure 4
Ligusticum wallichii extract inhibited the expression of TLR4, TRAF-6, NF-κB in myocardium. (a) Representative micrographs were taken at a magnification of ×400. The brown areas were TLR4 positive cell; the brown areas were significantly increased in the AMI model group. Ligusticum wallichii and aspirin decreased the brown area. (b) In the sham group, the immunoreactive staining occurred less in the cytoplasm. The number of TLR4-like immunoreactive cells increased significantly in the myocardium after AMI when compared with the sham group . Ligusticum wallichii (10 mg/Kg/d body weight) and aspirin (10 mg/Kg/d body weight) both inhibited TLR4 protein expression versus AMI group , but there were no remarkable differences between LW group and aspirin group. (c) The brown areas were TRAF-6 positive cell; the brown areas were significantly increased in the AMI model group. Ligusticum wallichii and aspirin decreased the brown area. (d) In the sham group, the immunoreactive staining occurred less in the cytoplasm. The number of TRAF-6-like immunoreactive cells increased significantly in the myocardium after AMI when compared with the sham group . Ligusticum wallichii and aspirin both inhibited TRAF-6 protein expression versus AMI group , but there were no remarkable differences between LW group and aspirin group. (e) The brown areas were NF-κB positive cell; the brown areas were significantly increased in the AMI model group. Ligusticum wallichii and aspirin decreased the brown area. (f) In the sham group, the immunoreactive staining occurred less in the cytoplasm. The number of NF-κB-like immunoreactive cells increased significantly in the cytoplasm and nucleus after AMI. Ligusticum wallichii and aspirin both inhibited NF-κB protein expression versus AMI group .
