Neurotrophic Signaling Factors in Brain Ischemia/Reperfusion Rats: Differential Modulation Pattern between Single-Time and Multiple Electroacupuncture Stimulation

Figure 3

Gene expression change patterns in MCAO rats with or without EA stimulation at days 1 and 7 of reperfusion. The mRNA expression changes were determined by GE array Q series Rat Neurotrophin and Receptors Gene Array. Genes with remarkable expression change (greater than 2.0-fold) were grouped in apoptosis-related genes (a), growth factors and receptors (b), neuropeptides and receptors (c), inflammatory-related factors (d), signaling molecules (e), and neurotrophins and receptors (f). (g) (single-time EA stimulation) and (h) (multiple EA stimulation) show the further network analysis of differentially expressed genes by Ingenuity Pathway Analysis; red color means upregulated moleculars, green color means downregulated moleculars, and white color means the moleculars close functionally connected within the network predicted by IPA. isc, ischemia group; sham, sham-operation group; and EA, ischemia plus EA group. Rep 1 d, day 1 of reperfusion; Rep 7 d, day 7 of reperfusion.