Tetramethylpyrazine Protects against Hydrogen Peroxide-Provoked Endothelial Dysfunction in Isolated Rat Aortic Rings: Implications for Antioxidant Therapy of Vascular Diseases

Table 4

DHE-mediated fluorescence microscopy assay in primary rat aortic endothelial cells.

Treatment	Fluorescence intensity (% of control)
Control	100 ± 0	7
H2O2 (200 M)	158 ± 1.16*	9
TMP (10 nM) + H2O2	86 ± 2.10^#	5
Apocynin (10 M) + H2O2	88.30 ± 2.25^#	4
DPI (10 nM) + H2O2	87.28 ± 5.40^#	3
Tiron (100 M) + DETCA (1 mM) + H2O2	67.95 ± 2.81^#	4

Data represent the means ± the SEM of 3–9 independent experiments ( versus control; versus H₂O₂).
DHE: dihydroethidium; DETCA: diethyldithiocarbamic acid; DPI: diphenyleneiodonium; H₂O₂: hydrogen peroxide; SEM: standard error of the mean; TMP: tetramethylpyrazine.