Evidence-Based Complementary and Alternative Medicine / 2014 / Article / Fig 6

Research Article

Antiplatelet Activity of Morus alba Leaves Extract, Mediated via Inhibiting Granule Secretion and Blocking the Phosphorylation of Extracellular-Signal-Regulated Kinase and Akt

Figure 6

Effects of Morus alba leaves extracts (MAE) on fibrinogen binding to integrin in ADP-activated platelets. The inhibitory effects of MAE on fibrinogen binding to integrin in collagen-activated platelets were measured by flow cytometry (A). Washed platelets (3 × 108/mL) were pretreated with vehicle (DMSO) or MAE at concentrations ranging from 100 μg/mL to 400 μg/mL. Collagen was then incubated with human fibrinogen labeled with Alexa Fluor 488 (20 μg/mL) for 5 min. The cells were subsequently fixed with 0.5% paraformaldehyde at 4°C for 30 min. Graphs showing fluorescent intensity present the data from one experiment but are representative of four independent trials. Data are expressed as the mean fluorescence intensity (MFI) of fibrinogen-positive platelets. Each graph presents the results expressed as percent of gated (a). ** compared to the agonist control.