Brazilian Red Propolis Induces Apoptosis-Like Cell Death and Decreases Migration Potential in Bladder Cancer Cells

Figure 2

Brazilian red propolis ethanolic extract increased antiproliferative and cytotoxicity effect in 5637 cells (a). Cell proliferation in 5637 and CHO-K1 was investigated by MTT assay. Data are expressed as means ± SEM from three independent experiments. Different letters (A/a, B/b, and C/c) indicate significant differences between the means. Uppercase letter indicates difference between the treatments in 5637 cells. Lowercase letter indicates difference between the treatments in CHO-K1 cells. The differences were considered significant at . VC = vehicle control (EtOH-H₂O). (b) Brazilian red propolis ethanolic extract increased 5637-cell death. Bladder cancer cells were treated with the BRP extract for 24 h. Analysis of cell death was estimated by LIVE/DEAD assay with 20x optical zoom. Untreated cells (A); 25 μg/mL treatment (B); 50 μg/mL treatment (C); 100 μg/mL treatment (D).

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