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Evidence-Based Complementary and Alternative Medicine
Volume 2014 (2014), Article ID 650905, 16 pages
http://dx.doi.org/10.1155/2014/650905
Research Article

Linking Bacterial Endophytic Communities to Essential Oils: Clues from Lavandula angustifolia Mill

1Trees and Timber Institute, National Research Council, Via Madonna del Piano, No. 10, Sesto Fiorentino, 50019 Florence, Italy
2Laboratory of Microbial and Molecular Evolution, Department of Biology, University of Florence, Via Madonna del Piano 6, Sesto Fiorentino, 50019 Florence, Italy
3Department of Neuroscience, Psychology, Drug Research and Child Health, University of Florence, Viale Pieraccini 6, 50139 Florence, Italy
4Center for Integrative Medicine, Careggi University Hospital, University of Florence, Viale Pieraccini 6, 50139 Florence, Italy
5Il giardino delle Erbe, via Del Corso 6, Casola Valsenio, 48010 Ravenna, Italy

Received 17 January 2014; Accepted 29 April 2014; Published 26 May 2014

Academic Editor: Gyorgyi Horvath

Copyright © 2014 Giovanni Emiliani et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplementary Figure 1: Bayesian dendrogram showing the relationships among the 16S rDNA sequences of 47 isolates belonging to the genus Bacillus and those of reference type strains. Posterior probability values are indicated at the node. Nodes are collapsed at 70% probability. LT = bacteria isolated from the rhizosphere, LR = bacteria isolated from the roots, LS = bacteria isolated from the stem.

Supplementary Figure 2: Bayesian dendrogram showing the relationships among the 16S rDNA sequences of isolates belonging to the genus Pseudomonas and those of reference type strains. Posterior probability values are indicated at the node. Nodes are collapsed at 70% probability. LT= bacteria isolated from the rhizosphere, LR = bacteria isolated from the roots, LS = bacteria isolated from the stem, LL = bacteria isolated from the leaves.

Supplementary Figure 3: Maximum parsimony dendrogram showing the relationships among the 16S rDNA sequences of 37 isolates belonging to the genus Stenotrophomonas and those of reference type strains. Bootstrap values are indicated at the node. LL= bacteria isolated from the leaves, LR = bacteria isolated from the roots (see Material and Methods and Suppl. Table 4 for details).

Supplementary Figure 4: Maximum parsimony dendrogram showing the relationships among the 16S rDNA sequences of 46 isolates belonging to the genus Rhizobium and those of reference type strains. Bootstrap values are indicated at each node. Nodes are collapsed at 70% probability. LT= bacteria isolated from the rhizosphere, LR = bacteria isolated from the roots (see Material and Methods and Suppl. Table 4 for details).

Supplementary Figure 5: Maximum parsimony dendrogram showing the relationships among the 16S rDNA sequences of 25 isolates belonging to the genus Pantoea and those of reference type strains. Bootstrap values are indicated at the node. Nodes are collapsed at 70% probability. LS = bacteria isolated from the stem, LL = bacteria isolated from the leaves (see Material and Methods and Suppl. Table 4 for details).

Supplementary Figure 6: Maximum parsimony dendrogram showing the relationships among the 16S rDNA sequences of 30 isolates belonging to the genus Microbacterium and those of reference type strains. Bootstrap values are indicated at the node. Nodes are collapsed at 70% probability. LT = bacteria isolated from the rhizosphere, LS = bacteria isolated from the stem, LL = bacteria isolated from the leaves (see Material and Methods and Suppl. Table 4 for details).

Supplementary Figure 7: Maximum parsimony dendrogram showing the relationships among the 16S rDNA sequences of 47 isolates belonging to the genus Bacillus and those of reference type strains. Bootstrap values are indicated at the node. Nodes are collapsed at 70% probability. LT = bacteria isolated from the rhizosphere, LR = bacteria isolated from the roots, LS = bacteria isolated from the stem (see Material and Methods and Suppl. Table 4 for details).

Supplementary Figure 8: Maximum parsimony dendrogram showing the relationships among the 16S rDNA sequences of isolates belonging to the genus Pseudomonas and those of reference type strains. Bootstrap values are indicated at the node. Nodes are collapsed at 70% probability. LT= bacteria isolated from the rhizosphere, LR = bacteria isolated from the roots, LS = bacteria isolated from the stem, LL = bacteria isolated from the leaves (see Material and Methods and Suppl. Table 4 for details).

Supplementary Figure 9: NJ dendrogram showing the relationships among the 16S rDNA sequences of 37 isolates belonging to the genus Stenotrophomans and those of reference type strains. Scale bars represent the Kimura-2 distance. Bootstrap values are indicated at the node. Nodes are collapsed at 70% probability. LL= bacteria isolated from the leaves, LR = bacteria isolated from the roots.

Supplementary Figure 10: NJ dendrogram showing the relationships among the 16S rDNA sequences of 46 isolates belonging to the genus Rhizobium and those of reference type strains. Scale bars represent the Kimura-2 distance. Bootstrap values are indicated at each node. Nodes are collapsed at 70% probability. LT= bacteria isolated from the rhizosphere, LR = bacteria isolated from the roots.

Supplementary Figure 11: NJ dendrogram showing the relationships among the 16S rDNA sequences of 25 isolates belonging to the genus Pantoea and those of reference type strains. Scale bars represent the Kimura-2 distance. Bootstrap values are indicated at the node. Nodes are collapsed at 70% probability. LS = bacteria isolated from the stem, LL = bacteria isolated from the leaves.

Supplementary Figure 12: NJ dendrogram showing the relationships among the 16S rDNA sequences of 30 isolates belonging to the genus Microbacterium and those of reference type strains. Scale bars represent the Kimura-2 distance. Bootstrap values are indicated at the node. Nodes are collapsed at 70% probability. LT = bacteria isolated from the rhizosphere, LS = bacteria isolated from the stem, LL = bacteria isolated from the leaves.

Supplementary Figure 13: NJ dendrogram showing the relationships among the 16S rDNA sequences of 47 isolates belonging to the genus Bacillus and those of reference type strains. Scale bars represent the Kimura-2 distance. Bootstrap values are indicated at the node. Nodes are collapsed at 70% probability. LT = bacteria isolated from the rhizosphere, LR = bacteria isolated from the roots, LS = bacteria isolated from the stem.

Supplementary Figure 14: NJ dendrogram showing the relationships among the 16S rDNA sequences of isolates belonging to the genus Pseudomonas and those of reference type strains. Scale bars represent the Kimura-2 distance. Bootstrap values are indicated at the node. Nodes are collapsed at 70% probability. LT= bacteria isolated from the rhizosphere, LR = bacteria isolated from the roots, LS = bacteria isolated from the stem, LL = bacteria isolated from the leaves.

Supplementary Table 1: List of bacterial endophytic strains used in this work as tester in the crossstreak experiments.

Supplementary Table 2: Number of Bcc target strains whose growth is inhibited by lavender bacterial endophytes.

Supplementary Table 3: Sensitivity spectrum exhibited by the 40 Bcc strains in the cross streak experiment vs a panel of lavender endophytic bacterial isolates.

Supplementary Table 4: Length of aligned matrices (bp), Variable and conserved characters number, Parsimony-informative characters, Consistency Index (CI), Retention Index (RI) and species used as outgroup of dendrograms obtained with Maximun Parsimony (see Materials and Methods for details).

  1. Supplementary Material