Ethyl Acetate Extract of Artemisia anomala S. Moore Displays Potent Anti-Inflammatory Effect

Figure 3

Effect of pretreatment and posttreatment of EAFA on NO production and cell viability. Cells were plated at a density of 1×10⁵ cells/well in a 96-well plate and allowed to attach for 2 h. EAFA was added prior to (pretreatment-12 h, pretreatment-6 h), simultaneously with (0 h) (a, b) or after the treatment of the cells with IFN𝛾 (10 U/mL) plus LPS (100 ng/mL) (posttreatment-6 h, posttreatment-12 h) (c, d). Nitric concentrations in the culture medium and cell viability were determined by the Griess reaction and MTT assay. The values (means ± SEM) were obtained from three independent experiments. ^##𝑃<0.01; ^#𝑃<0.05 versus control group; **𝑃<0.01; *𝑃<0.05  versus model group.

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