Ethyl Acetate Extract of Artemisia anomala S. Moore Displays Potent Anti-Inflammatory Effect
Figure 6
Effect of EAFA on NF-B and MAPK activities in LPS/IFN-stimulated RAW264.7 cells. (a, b) DNA binding activity of p50 and p65 proteins in nuclear extracts was assessed using NF-Bp50/p65 EZ-TFA transcription factor assay. Absorbance was measured at 450 nm in a microplate spectrophotometer. Results were normalized to absorbance/mg protein. The data shown are representative of three independent experiments. ##, # versus control group; **, * versus model group. (c) RAW264.7 cells were plated at a density of cells/well in 30 mm dish for overnight. EAFA was added to cells for 1 h followed by 30 min stimulation of IFN (10 U/mL) plus LPS (100 ng/mL). Whole cell lysates were prepared and subjected to western blotting to detect phosphor-ERK, phosphor-JNK, and phosphor-p38 with the respective antibodies. Data shown are the representative of three independent experiments. ##, # versus control group; **, * versus model group.
