New Treatment for Alzheimer’s Disease, Kamikihito, Reverses Amyloid--Induced Progression of Tau Phosphorylation and Axonal Atrophy
Figure 3
Effect of KKT and KPL pre- and posttreatment on A-induced axonal atrophy. (a) For pretreatment, cortical neurons were cultured for 3 days and then treated with vehicle solution, KKT (10 μg/mL), or KPL (1 μM). After 1 h, the cells were treated with A (25–35) (10 μM) for 24 h, and A was removed from the medium. Each drug treatment was continued for 72 h. (b) For posttreatment, cortical neurons were cultured for 3 d and then treated with A (25–35) (10 μM). At 24 h after A treatment, A was removed from the medium and the cells were treated with vehicle solution, KKT (10 μg/mL), or KPL (1 μM) for 72 h. After each drug treatment, the cells were fixed and immunostained with an antibody against phosphorylated NF-H and MAP2. The lengths of phosphorylated NF-H-positive neurites were quantified for each treatment. (, One-way ANOVA, post hoc Bonferroni’s multiple comparison test).
