Lycopene Inhibits Urotensin-II-Induced Cardiomyocyte Hypertrophy in Neonatal Rat Cardiomyocytes
Figure 4
Lycopene treatment prevents Akt/GSK-3β phosphorylation in U-II-stimulated neonatal rat cardiomyocytes. Protein extracts from cardiomyocytes were subjected to immunoblot analysis. (a) Effects of lycopene on U-II-induced Akt phosphorylation in cardiomyocytes. Upper panels: representative immunoblots using total and antiphospho-Akt (Ser473) antibodies. Lower panels: the indicated values represent the ratio of phosphorylated protein to total protein. (b) Effects of lycopene on U-II-induced GSK-3β phosphorylation in cardiomyocytes. Upper panels: representative immunoblots using total and antiphospho-GSK-3β (Ser9) antibodies. Lower panels: the indicated values represent the ratio of phosphorylated protein to total protein. Neonatal rat cardiomyocytes were nonstimulated (control) or stimulated with U-II (1 nM for 2 min). When indicated, lycopene (3 μM) was added 6 h before U-II stimulation. The results are shown as mean ± SEM . versus control; versus U-II alone.
