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Figure 4: (a, c) Effects of decoctions on mRNA levels of SUR1 and Kir6.2 measured by real-time PCR. The fold changes of SUR1 and Kir6.2 expressions are calculated by the method and expressed as mean fold induction over control group that has been normalized to 1.0. Data are expressed as mean ± SEM (, versus control group, versus model group). DBYW: Da-Bu-Yin-Wan; QZS: Qian-Zheng-San; CD: combined decoction. (b, d) Melting curve analysis of SUR1 and Kir6.2. Tm: temperature of melting.