Antioxidant and Antiadipogenic Activities of Galkeun-Tang, a Traditional Korean Herbal Formula

Figure 4

Inhibitory effect of GKT extract on triglyceride accumulation in 3T3-L1 adipocytes. 3T3-L1 preadipocytes were differentiated into adipocytes by adding isobutylmethylxanthine, dexamethasone, and insulin (MDI) for 8 days. The cells were treated with or without GKT or GW9662 (20 μM) during the differentiation period. (a and b) Lipid accumulation in the cells was analyzed by Oil Red O staining. (a) The cells stained with Oil Red O were visualized using an Olympus CKX41 inverted microscope at ×200 magnification (left panel). Stained oil droplets were dissolved in isopropyl alcohol and measured by reading absorbance at 520 nm (right panel). (b) The content of triglyceride was enzymatically measured at 570 nm using a commercial kit (BioVision Inc., Milpitas, CA). Data are presented as the mean ± S.E.M. and compared with the differentiated control.

(a)

(b)