Crotonis Fructus and Its Constituent, Croton Oil, Stimulate Lipolysis in OP9 Adipocytes

Figure 1

Effects of Crotonis Fructus extracts (CEF) and croton oil (CO) on glycerol release and viability. Differentiated OP9 adipocytes were treated with various concentrations of CFE (20–50 μg/mL), CO (2–20 μg/mL), and 200 nM isoproterenol (ISO) for 12 h. Glycerol release (black bar) was determined with a glycerol quantification kit and cell viability (gray bar) was quantified with MTT assay as described in Materials and Methods. Data are expressed as the mean ± SD of four independent experiments and a percentage of vehicle (DMSO-)treated control cells (CON). ^*, ^** versus CON.

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