The Rhizome Mixture of Anemarrhena asphodeloides and Coptidis chinensis Ameliorates Acute and Chronic Colitis in Mice by Inhibiting the Binding of Lipopolysaccharide to TLR4 and IRAK1 Phosphorylation
Figure 5
Inhibitory effect of AC-mix, AA, CC, mangiferin, and berberine on the activation of IRAK1, IKKβ, and NF-κB and the expression of TNF-α, IL-1β, and IL-6 in LPS-stimulated peritoneal macrophages. (a) Effect on the activation of IRAK1, IKKβ, and NF-κB. Test agents were treated for 90 min in LPS-stimulated macrophages and then the proteins were measured by immunoblotting. (b) Effect on the expression of TNF-α, IL-1β, and IL-6. Test agents (AC, AA, CC, mangiferin and berberine) were treated for 24 h min in LPS-stimulated macrophages and then the cytokines were measured by ELISA. (c) Cell viability, measured by tryphan blue staining assay. Test agents [AC-mix, 20 μg/mL; AA, 20 μg/mL; CC, 20 μg/mL; mangiferin, 20 μM; and berberine, 20 μM] were treated for 2 days. All data are expressed as mean ± S.D. ( in a single experiment). different versus group treated with LPS alone (). different versus LPS-nontreated group ().
