Research Article

Multifunctional Effects of Mangosteen Pericarp on Cognition in C57BL/6J and Triple Transgenic Alzheimer’s Mice

Table 1

List of the primary antibodies used in this study.

AntibodiesSpeciesSupplierWB dilutionIHC dilutionEpitope specificity

Amyloid beta antibodies
 ARabbitInvitrogen1 : 2,000Amyloid beta
 ARabbitInvitrogen1 : 500Amyloid beta
 APPRabbitSigma-Aldrich1 : 500Amyloid precursor protein
 BACERabbitCell Signaling1 : 1,000Beta secretase
Neurotransmission antibodies
 5-HTRatMillipore1 : 100Serotonergic neurons
 CalbindinMouseSigma-Aldrich1 : 1,000Calcium binding protein
 ChATRabbitMillipore1 : 500Cholinergic neurons
 NeuNMouseMillipore1 : 1,000
1 : 500 (IF)
Neuronal cells
 NR2ARabbitMillipore1 : 1,000NMDA receptor 2A
 NR2BRabbitMillipore1 : 1,000NMDA receptor 2B
 THRabbitMillipore1 : 1,000Noradrenergic neurons
Inflammation antibodies
 COX2RabbitMillipore1 : 1,000Prostaglandin synthase-2
 GFAPMouseMillipore1 : 1,000
1 : 500 (IF)
Astrocytes
 Iba-1RabbitWako1 : 1,000Microglia
Signaling antibodies
 Akt 1/2RabbitCell Signaling1 : 1,000Total Akt
 pAkt 1/2RabbitCell Signaling1 : 1,000Akt phosphorylated at Thr450
 CDK5MouseMillipore1 : 1,000Cyclin-dependent kinase-5
 Erk 1/2RabbitCell Signaling1 : 1,000Total Erk 1/2
 pErk 1/2RabbitCell Signaling1 : 1,000Erk 1/2 phosphorylated at Thr202/Tyr204
 GSK3RabbitCell Signaling1 : 1,000Total GSK3
 pGSK3RabbitCell Signaling1 : 1,000GSK3 phosphorylated at Ser21
 GSK3RabbitEpitomics1 : 1,000Total GSK3
 pGSK3RabbitEpitomics1 : 1,000GSK3 phosphorylated at Ser9
 JNKRabbitCell Signaling1 : 1,000Total JNK
 pJNKRabbitCell Signaling1 : 1,000JNK phosphorylated at Thr183/Tyr185
 p38RabbitCell Signaling1 : 1,000Total p38
 pp38RabbitCell Signaling1 : 1,000p38 phosphorylated at Thr180/Tyr182
Tau antibodies
 pTauRabbitAnaSpec1 : 1,000Tau hyperphosphorylated at Ser202
 pTauRabbitMillipore1 : 1,000Tau hyperphosphorylated at Ser262
 Tau1MouseMillipore1 : 1,000Tau unphosphorylation form
Other antibodies
-ActinMouseMillipore1 : 2,000-Actin
 BDNFRabbitMillipore1 : 500 (IF)Brain-derived neurotrophic factor

WB: Western blot; IHC: immunohistochemistry; IF: immunofluorescence.