Inhibitory Effect on β-Hexosaminidase Release from RBL-2H3 Cells of Extracts and Some Pure Constituents of Benchalokawichian, a Thai Herbal Remedy, Used for Allergic Disorders

Table 5

Anti-allergic activity of the ethanolic extract, fractions and pure constituents from Benchalokawichian remedy.

Samples	%Inhibition at various concentrations						IC50 of Antiallergic activity
Crude, semipure extracts, and pure compounds	0.1	1	5	10	50	100	IC50  ±  SEM, μg/mL
							(μM data for pure compound)
Ficus racemosa Linn.	—	15.0 ± 10.5	—	28.9 ± 9.1	63.6 ± 1.6	75.8 ± 4.3	27.1 ± 1.6
Capparis micracantha DC.	—	4.2 ± 2.7	—	9.5 ± 7.1	22.9 ± 8.9	32.3 ± 6.3	>100
Tiliacora triandra Diels.		−24.48 ± 5.7		−0.0 ± 1.8	11.7 ± 4.8	44.2 ± 4.6	>100
Harrisonia perforata Merr.	—	21.6 ± 5.3	—	42.4 ± 1.3	62.8 ± 0.0	78.5 ± 0.8	14.5 ± 0.1
Clerodendrum petasites S. Moore		−6.3 ± 5.1		7.9 ± 2.2	44.0 ± 1.4	85.0 ± 1.9	57.8 ± 1.4
Benchalokawichian (Ha-Rak)	—	9.7 ± 5.5	—	24.2 ± 3.6	52.1 ± 1.5	45.2 ± 5.1	39.8 ± 1.3
Fraction 1; hexane (F1)	—	—	—	—	—	—	NT
Fraction 2; hexane : chloroform (F2)	—	22.5 ± 0.4	—	30.4 ± 2.1	43.6 ± 0.8	53.0 ± 0.7	86.1 ± 1.1
Fraction 3; chloroform (F3)	—	23.1 ± 4.5	—	40.3 ± 3.4	59.5 ± 4.4	71.2 ± 1.7	17.9 ± 3.0
Fraction 4; chloroform : methanol (F4)	—	20.9 ± 7.7	—	38.9 ± 3.9	58.2 ± 2.7	70.5 ± 3.7	19.6 ± 3.4
Fraction 5; methanol (F5)	—	10.5 ± 7.6	—	26.0 ± 6.2	52.1 ± 1.5	63.9 ± 2.9	39.9 ± 1.9
Compound 1; pectolinarigenin	17.1 ± 1.7	25.1 ± 3.9	38.6 ± 7.2	101.1 ± 9.5	—	—	6.3 ± 0.7 (20.1 μM)
Compound 2; O-methylalloptaeroxylin	—	−15.8 ± 2.3	18.9 ± 0.9	40.5 ± 0.0	—	91.9 ± 1.6	14.2 ± 0.9 (51.8 μM)
Chlorpheniramine^a	—	4.0 ± 1.9	—	35.1 ± 0.8	44.4 ± 1.8	81.1 ± 3.2	16.2 ± 2.5 (58.8 μM)

Positive control.
NT (—) means not tested.