Anti-Inflammatory and Antihyperalgesic Activities of Ethanolic Extract and Fruticulin A from Salvia lachnostachys Leaves in Mice

Figure 4

Effect of oral administration of SLEE at inhibition of the leukocyte migration (a) and protein extravasation (b) on pleurisy test in mice. Mice were treated one hour before an intrapleural injection of carrageenan, with SLEE (30, 100, and 300 mg/kg p.o.), dexamethasone (DEXA, 1 mg/kg, s.c.), or saline solution (V). Naive group (N), also treated with saline p.o., received an intrapleural injection of sterile saline. Pleural cavity was washed with PBS/EDTA 10 mM. Cells were counted and plasma leakage was analyzed. In (a), number of cells that migrated to pleural cavity 4 hours after carrageenan injection. In (b), plasma leakage measured by Bradford’s reaction. The bars express the mean ± SEM of 5 animals. The symbol compared vehicle (V) versus SLEE or DEX treated groups and compared the vehicle treated group with naive. ; ; . Differences between groups were analyzed by analysis of variance (one-way ANOVA) followed by the Newman-Keuls test.

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