Beneficial Effects of Fractions of Nardostachys jatamansi on Lipopolysaccharide-Induced Inflammatory Response
Figure 5
Effects of NJ fractions on MAPKs activation. ((a)–(d)) Peritoneal macrophages were treated with NJ fractions (100 μg/mL) for 1 h and then stimulated with LPS (500 ng/mL) at indicated time points. Cells were harvested for western blot. Total cellular proteins (20 μg) were resolved by SDS-PAGE, transferred to PVDF membrane, and detected with phospho-specific ERK 1/2 (42/44 kDa), JNK (46/54 kDa), and p38 (43 kDa) antibody. ERK, JNK, and p38 were used as a loading control. A representative western blot of three experiments is shown.
