Research Article

Combination of Paeoniae Radix and Cnidii Rhizoma Prolonged Survival of Fully Mismatched Cardiac Allografts and Generated Regulatory Cells in Mice

Figure 1

Graft survival of CBA mice given oral administration of agents and histologic studies. (a) Recipients with C57BL/6 hearts were either untreated, given distilled water, or given 2 g/kg/day of TJ-23 from the day of transplantation until 7 days afterward. MST: median survival time; # and * for difference between 2 groups. (b) CBA recipients with C57BL/6 hearts were treated with mixture of two agents (2 g/kg/day of Poria sclerotium, Cnidii rhizoma, and Paeoniae radix) from the day of transplantation to 7 days afterward. MST, median survival time; * for difference between 2 groups; NS = no significant difference. (c) CBA recipients with C57BL/6 hearts were treated with various amounts of mixtures of Paeoniae radix and Cnidii rhizoma. MST: median survival time; * for difference between 2 groups. (d) Histologic studies of harvested cardiac allografts stained with hematoxylin-eosin (HE). The upper left picture shows a representative sample obtained from mice given mixture of Paeoniae radix and Cnidii rhizoma and the lower left picture shows the sample from untreated mice (magnification ×20 of two pictures). The right graph shows the degree of mononuclear cell infiltration was assessed by grading with semiquantitative scale (0, no infiltration; 1, faint and limited infiltration; 2, moderate infiltration; 3, severe infiltration). *** for difference between 2 groups.
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