Research Article

Combination of Paeoniae Radix and Cnidii Rhizoma Prolonged Survival of Fully Mismatched Cardiac Allografts and Generated Regulatory Cells in Mice

Figure 2

Evidence of generation of regulatory cells in CBA allograft recipients treated with combination of Paeoniae radix and Cnidii rhizoma. (a) Cardiac allograft survival after adoptive transfer of whole splenocytes. MST: median survival time; * for difference between 2 groups. (b) CD4, CD25, and Foxp3 expression in splenocytes, as determined by flow cytometry. The upper panels show representative data of dot plots on flow cytometry and the percentage of CD4+CD25+Foxp3+ in CD4+ cells from splenocytes in no treatment and combination of Paeoniae radix and Cnidii rhizoma. Data are mean ± SD ( mice in each group). *** for difference between 2 groups. (c) Infiltration of Foxp3+ cells in cardiac grafts 14 days after grafting from untreated or mixture-treated recipients (magnification ×20). The right graph shows the count of infiltrating Foxp3+ cells in cardiac allografts form untreated or mixture-treated recipients. # for difference between 2 groups.
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