Research Article

Combination of Paeoniae Radix and Cnidii Rhizoma Prolonged Survival of Fully Mismatched Cardiac Allografts and Generated Regulatory Cells in Mice

Figure 3

Evidence of induction of alloproliferative hyporesponsiveness in CBA recipients of allograft treated with combination of Paeoniae radix and Cnidii rhizoma. (a) Results of cell-proliferation assays in mixed leukocyte cultures (MLCs). The data shown are mean ± SD values derived from samples from 6 mice in each group. * for difference between 2 groups. NS: no significant difference. (b) and (c) Levels of cytokines in MLCs. Levels of interferon-γ (b) and interleukin 4 (IL-4) (c) in the MLCs were assessed by enzyme-linked immunosorbent assays. Data are shown as mean ± SD values derived from samples from 6 mice in each group. * for difference between 2 groups. (d) Direct effect of combination of Paeoniae radix and Cnidii rhizoma on alloproliferation in MLC. The data shown are mean ± SD values derived from samples from 6 mice in each group. * for difference between 2 groups. NS: no significant difference.
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