Figure 5: Chromatography fractionation of maggot ES SPE eluate. Maggot ES SPE eluate was fractionated by HPLC system using C18 column ( ; 5 μm) at a flow rate 0.3 mL/min, by using a gradient from 0 to 90% (v/v) acetonitrile (containing 0.1% (v/v) trifluoroacetic acid), during 70 min (a). Fractions with antibiofilm activity against S. aureus established biofilm were then fractionated by C4 column with the same conditions (b) and checked for its activity against Staphylococcus aureus established biofilm. PC is positive control, established biofilm without ES treatment.