Evidence-Based Complementary and Alternative Medicine / 2014 / Article / Fig 6

Research Article

Electroacupuncture Promotes Proliferation of Amplifying Neural Progenitors and Preserves Quiescent Neural Progenitors from Apoptosis to Alleviate Depressive-Like and Anxiety-Like Behaviours

Figure 6

EA relieves the apoptosis of QNPs in DG of the stressed rats. ((a)–(h)) Representative GFAP (red), Sox2 (green), and Hoechst (blue) triple-stained sections show that typical cells are undergoing apoptosis or not in different types. The Hoechst staining is below the merged triple staining in every figure to show the typical apoptotic morphology of the nucleus (pyknosis, deep into dense) or normal nucleus (without any nuclear condensation and fragmentation). (a) A normal QNP (arrow). (b) An apoptotic QNP (arrow). (c) A normal ANP (arrow). (d) An apoptotic ANP (arrow). (e) A normal astrocyte (arrow). (f) An apoptotic astrocyte (arrow). (g) Normal granular cells in GCL (arrows). (h) Apoptotic granular cells in GCL (arrows). Bar gram in panel (i) depicts (mean ± SE) the quantification of the different types of the apoptotic cells in the whole DG. EA protects the stressed rats when they exhibits an antiapoptotic effect on the QNPs in DG. and # , versus Model group.
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