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Evidence-Based Complementary and Alternative Medicine
Volume 2014 (2014), Article ID 878503, 12 pages
Research Article

Synergistic Effect of Fermented Rice Extracts on the Probiotic and Laxative Properties of Yoghurt in Rats with Loperamide-Induced Constipation

1RIS Center, IACF, Silla University, Gwaebup-dong, Sasang-gu, Busan 617-736, Republic of Korea
2Department of Bio-Food Materials, Silla University, Sasang-gu, Busan 617-736, Republic of Korea
3Glucan Corp. No. 305 Marine Bio-Industry Development Center, Hoenggye-ri 27, Ilgwang-myeon, Gijan-gun, Busan 619-912, Republic of Korea
4JKnutra, No. 502, 17 Joongdaero 23-gil, Songpa-ku, Seoul 138-160, Republic of Korea
5Department of Anatomy and Histology, College of Oriental Medicine, Daegu Haany University, 290 Yugok-dong, Gyeongsan-si, Gyeongsanbuk-do 712-715, Republic of Korea

Received 8 April 2014; Revised 10 July 2014; Accepted 17 July 2014; Published 20 August 2014

Academic Editor: I-Min Liu

Copyright © 2014 Jae-Suk Choi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Aim. The objective was to evaluate the synergistic effects of fermented rice extracts (FRe) on the laxative and probiotic properties of yoghurt in rats with loperamide-induced constipation. Methods. After constipation induction, yoghurt containing FRe (BFRe; 0.05%, 0.1%, or 1%) was administered orally once per day for 6 days. Results. Loperamide treatment caused marked decreases in fecal pellet numbers and water content discharged, as well as in the surface mucosal thickness of the colonic lumen, intestinal charcoal transit ratio, thickness, and number of mucous-producing goblet cells in the colonic mucosa, whereas it increased the remnant fecal pellet number and the mean diameter of the colonic lumen. However, this loperamide-induced constipation was ameliorated by treatment with FRe, yoghurt single formula, or 0.05%, 0.1%, or 1% BFRe (10 mL/kg). Additionally, the viable numbers of Lactobacillus in the cecal contents and feces were markedly higher than those in constipated rats. Moreover, greater probiotic and laxative effects were detected in BFRe-treated rats than in rats treated with equivalent doses of yoghurt or FRe single formula. Conclusion. The results suggest that addition of FRe to liquid yoghurt will enhance the probiotic and beneficial laxative effects of yoghurt in the digestive tract, without causing side effects.