Inhibition of Adipogenesis by Oligonol through Akt-mTOR Inhibition in 3T3-L1 Adipocytes
Figure 1
Effect of Oligonol on 3T3-L1 adipocytes. (a) Cell viability of 3T3-L1 preadipocytes and differentiated adipocytes treated with Oligonol (OLG). 3T3-L1 preadipocytes were incubated with various concentrations of Oligonol (0–500 μg/mL) for 24 hrs and cell viability was measured by MTT assay. 3T3-L1 preadipocytes were differentiated into adipocytes with the MDI (mixture of IBMX, dexamethasone, and insulin)—containing media for 8 days. Cells were treated with 10, 25, or 50 μg/mL of Oligonol during entire differentiation period (from day 0 to day 8) or after induction of differentiation (from day 4 to day 8). (b) Lipid accumulation was determined by Oil Red O staining. (c) Cellular lipid accumulation was quantified by measuring the absorbance at 500 nm. Values are mean ± SEM of three independent experiments carried out in triplicates. *, ** compared with untreated control.
