Inhibition of Adipogenesis by Oligonol through Akt-mTOR Inhibition in 3T3-L1 Adipocytes
Figure 4
Effect of Oligonol on lipid biosynthesis and lipolysis in 3T3-L1 adipocytes. 3T3-L1 preadipocytes were differentiated into adipocytes with the absence or presence of Oligonol (10, 25, or 50 μg/mL) for 8 days. (a) Oligonol reduced TG content during differentiation of 3T3-L1 adipocyte. 3T3-L1 preadipocytes were differentiated in the absence or presence of Oligonol for 8 days, and the lipid accumulation was measured by triglyceride assay. (b) Oligonol inhibited the mRNA expressions of lipogenic gene, ACC, FAS, and SCD during differentiation. The gene expressions were analyzed by quantitative RT-PCR. All gene expressions were normalized using GAPDH as reference gene. (c) Protein expression levels of phospho-ACC, ACC, and FAS were analyzed by Western blot analysis in 3T3-L1 adipocytes treated with Oligonol. Cell lysates were collected from 3T3-L1 cells at days 4 and 8 after induction of adipocyte differentiation. (d) Glycerol release into the medium was quantified in fully differentiated 3T3-L1 adipocytes treated with Oligonol (10, 25, or 50 μg/mL). Values are mean ± SEM of three independent experiments carried out in triplicates. *, **, *** compared with untreated adipocytes.
