Ananas comosus L. Leaf Phenols and p-Coumaric Acid Regulate Liver Fat Metabolism by Upregulating CPT-1 Expression
Figure 8
Effect of PLP and CA on CPT-1 expression in oleic acid-untreated ((a) and (c)) and oleic acid-treated ((b) and (d)) HepG2 cells based on RT-PCR and Western blot assays. BSA: bovine serum albumin-treated blank control; DMSO: DMSO-treated control cells; PLP-10, PLP-50: pineapple leaf phenol-treated (final concentrations of 10 and 50 μg/mL, resp.) cells; CA-1 and CA-10: coumaric acid-treated (final concentrations of 1 and 10 μg/mL, resp.) cells; FB-1.4: fenofibrate-treated (final concentration of 1.4 μg/mL) cells; and +/−: incubation with (+) or without (−) oleic acid at the final concentration of 0.6 μM. Data are expressed as mean ± SD . #, ## versus BSA controls; *, ** versus DMSO controls.
