Effects of 6-Hydroxyflavone on Osteoblast Differentiation in MC3T3-E1 Cells
The activation of AKT, ERK, and JNK signaling in MC3T3-E1 cells by 6-hydroxyflavone (6-OH-F) and 7-hydroxyflavone (7-OH-F). Time-related effects of 6-OH-F and 7-OH-F on the phosphorylation of AKT, ERK, and JNK in MC3T3-E1 cells were analyzed using western blotting. The MC3T3-E1 cells treated with 20 μM 6-OH-F or 7-OH-F were collected at designated time points up to 60 min. The patterns of phosphorylated AKT, ERK1/2, and JNK in MC3T3-E1 cells were assessed at 5 min, 10 min, 15 min, 30 min, and 60 min after treatment with 6-OH-F (a) or 7-OH-F (b).