Research Article

Si-Jun-Zi Decoction Treatment Promotes the Restoration of Intestinal Function after Obstruction by Regulating Intestinal Homeostasis

Figure 3

Si-Jun-Zi Decoction treatment regulates the intestinal immune system. (a) Intestinal segments from the ileum were excised and fixed in 4% paraformaldehyde and embedded in paraffin blocks. We stained 4 μm thick sections with PAS, and the percentage of LPLs was quantified in photomicrographs of the intestines. (b) The level of s-IgA in the intestinal lumen was examined by ELISA. (c)–(f) The PP lymphocytes were isolated and the percentage of T cells subtypes was analyzed by flow cytometry. The percentage of CD3+T cells (c), the percentage of CD4+T cells (d), the percentage of CD8+T cells (e), and the CD4+/CD8+T cells ratio (f) are shown. The data are representative of three independent experiments, with each using 4-5 mice per group. Data are shown as mean ± SD ( ).
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