Proteomic Identification of Nrf2-Mediated Phase II Enzymes Critical for Protection of Tao Hong Si Wu Decoction against Oxygen Glucose Deprivation Injury in PC12 Cells

Figure 4

Discovery of Nrf2-mediated phase II enzymes induced by THSWD. (a) Enlargement of protein spots corresponding to Nrf2-driven proteins. The arrows indicate the target protein spots in THSWD-treated and control samples. (b) Quantitative real-time PCR analysis of the six genes corresponding to the 6 proteins directly regulated by Nrf2. After a 24 h induction by THSWD at indicated doses, SOD1 (A), Srx (B), GSTa2 (C), GCLM (D), NQO1 (E), and HO-1 (F) mRNA expression were determined by the quantitative real-time PCR system. Data represent the mean value of two biological replicates. Values represent mean ± SD (𝑛=3). ^∗𝑃<0.05, ^∗∗𝑃<0.01, and ^∗∗∗𝑃<0.001 versus vehicle control.

(a)

(b)