Proteomic Identification of Nrf2-Mediated Phase II Enzymes Critical for Protection of Tao Hong Si Wu Decoction against Oxygen Glucose Deprivation Injury in PC12 Cells

Table 1

Primers used for quantitative real-time PCR.

Gene	Product length	Primer sequences
SOD-1	129 bp	F	5′-CCACGAGAAACAAGATGACT-3′
		R	5′-GACTCAGACCACATAGGGAAT-3′
Srx	107 bp	F	5′-GACGTCCTCTGGATCAAAG-3′
		R	5′-GCAGGAATGGTCTCTCTCTG-3′
GSTa2	231 bp	F	5′-GGCAAAAGACAGGACCAAAA-3′
		R	5′-GGCTGCAGGAACTTCTTCAC-3′
GCLM	270 bp	F	5′-CTGACATTGAAGCCCAGGAG-3′
		R	5′-ACATTGCCAAACCACCACA-3′
NQO1	190 bp	F	5′-CGCAGAGAGGACATCATTCA-3′
		R	5′-CGCCAGAGATGACTCAACAG-3′
HO-1	107 bp	F	5′-ACCCCACCAAGTTCAAA CAG-3′
		R	5′-GAGCAGGAAGGCGGTCTTAG-3′
β-Actin	231 bp	F	5′-GGGGTGTTGAAGGTCTCAAA-3′
		R	5′-TGTCACCAACTGGGACGATA-3′

F: forward primer; R: reverse primer; SOD-1: superoxide dismutase [Cu–Zn]; Srx: sulfiredoxin; GSTa2: glutathione S-transferase alpha-2; GCLM: glutamate cysteine ligase, modifier subunit; NQO1: NAD(P)H quinone oxidoreductase 1; HO-1: heme oxygenase 1.