Proteomic Identification of Nrf2-Mediated Phase II Enzymes Critical for Protection of Tao Hong Si Wu Decoction against Oxygen Glucose Deprivation Injury in PC12 Cells
Table 2
MALDI/MS identification of the protein spots in response to THSWD.
Spot number
Fold change
Ther. /pIa
Protein description
Accession number
Pep. numberb
Score
120
1.3
15912/5.88
Superoxide dismutase [Cu–Zn]
P07632
3
89
145
1.6
15145/5.65
Beta-actin FE-3
Q99NC6
1
50
146
1.7
21592/4.92
Heat shock protein beta-8
Q9EPX0
1
88
148
1.4
14194/6.30
Sulfiredoxin
B3DM86
3
102
165
−1.2
22254/9.19
Major prion protein
P13852
2
58
170
−1.6
21249/5.00
rCG50690
EDL86882
1
43
186
32711/4.62
Nucleophosmin
P13084
1
77
188
3
18561/6.27
Glial fibrillary acidic protein epsilon
A1E252
1
52
202
1.5
25428/8.88
Glutathione S-transferase alpha-2
P04903
2
75
378
−1.3
36412/8.33
Protein phosphatase 1 regulatory subunit 3C
Q5U2R5
3
87
412
1.3
30548/5.36
Glutamate-cysteine ligase regulatory subunit
P48508
2
70
442
1.8
74099/5.97
Pre-mtHSP70
P48721
1
102
483
1.2
30868/9.34
NAD(P)H dehydrogenase [quinone] 1
P05982
2
83
511
1.3
33006/6.09
Heme oxygenase 1
P06762
2
75
561
1.3
32622/6.42
Stanniocalcin-2
Q9R0K8
563
1.3
40927/4.81
Protooncogene c-Fos
P12841
3
116
734
1.3
41643/5.31
Actin, gamma-enteric smooth muscle
P63269
2
95
737
1.3
42606/8.62
Early growth response protein 3
P43301
3
70
738
1.3
43657/6.41
Proliferation-associated 2G4
Q6AYD3
6
74
809
1.6
32894/4.86
Heterogeneous nuclear ribonucleoprotein C (C1/C2)
G3V9R8
3
136
849
38409/5.2
Suppressor of G2 allele of SKP1 homolog
B0BN85
2
53
1054
−2.4
50136 /4.9
Tubulin alpha-1A chain
P68370
2
100
1081
1.3
56573/8.6
G protein-activated inward rectifier potassium channel 1
P63251
4
167
1140
1.5
57926/5.35
60 kDa heat shock protein, mitochondrial
P63039
3
152
1399
−1.5
86990/7.62
Protein Igdcc3
D3ZQ86
4
129
1642
−1.3
108803/5.54
Ephrin type-A receptor 7
P54759
5
147
Theoretical molecular weight (kDa) and pI from the ExPASy database.
bThe number of unique peptides identified by MS/MS sequencing.
