Prolonged Diuretic Activity and Calcium-Sparing Effect of Tropaeolum majus: Evidence in the Prevention of Osteoporosis
Figure 3
HETM induces calcium-sparing effects without affecting osteocalcin levels in OVX rats. Different groups of rats were treated for 4 weeks with HETM (3, 30, and 300 mg/kg), furosemide (C+, 25 mg/kg), or vehicle (C−). On the first day of treatment (outset) and at weekly intervals for four weeks the urine was collected and calcium concentration (b) was measured. At the end of the experiment the animals were euthanized and their serum was collected for the determination of serum osteocalcin (a). Each bar represents the mean of 7 animals and the vertical lines show the S.E.M. denotes significance levels compared with the group treated with vehicle alone. One- (a) or two-way (b) ANOVA followed by Bonferroni test ( ).
