Screening to Identify Commonly Used Chinese Herbs That Affect ERBB2 and ESR1 Gene Expression Using the Human Breast Cancer MCF-7 Cell Line
Figure 2
Effects of commonly used Chinese herbal extracts on ESR1-luciferase activity using the MCF-7 cell line. The MCF-7 line (1 × 105 cells/well) was seeded for 24 h, followed by transfection with the ESR1-luciferase plasmid and treated with twenty-two single herbal extracts (a) and six compound recipes (b) as described in Section 2. The codes of twenty-two herbs (from A to V) are described in Section 2. Data are presented as the relative optic density ratio, namely, the ratio of luciferase activity to Renilla luciferase ratio and were analyzed using four to six independent experiments. An asterisk indicates a value < 0.05 versus the vehicle group by one-way ANOVA.
