Screening to Identify Commonly Used Chinese Herbs That Affect ERBB2 and ESR1 Gene Expression Using the Human Breast Cancer MCF-7 Cell Line
Figure 4
Effects of Ligusticum chuanxiong extract on ERα and HER2 protein expression using the MCF-7 cell line. The MCF-7 line (1 × 106 cells) was seeded for 24 h and changed to 1% CDFBS medium for 4 days and then treated with Suan-Zao-Ren-Tang (SZRT), the reduced form of Suan-Zao-Ren-Tang (r-SZRT) or Ligusticum chuanxiong extracts, for another 24 hr. Then the cells were treated with lysing buffer and analyzed by Western blotting using anti-HER2 antibodies (a) and anti-ERα antibody (b) as described in the Methods section. The OD ratio indicates the relative optic density ratio using β-actin as the control.
