Screening to Identify Commonly Used Chinese Herbs That Affect ERBB2 and ESR1 Gene Expression Using the Human Breast Cancer MCF-7 Cell Line
Figure 5
Effects of combined treatment with Ligusticum chuanxiong and estrogen (E2) on the cell proliferation of MCF-7 cells. The MCF-7 line (1 × 106 cells) was seeded for 24 h and changed to 1% CDFBS medium for 4 days and then treated with Ligusticum chuanxiong extract (1 μg/mL) with or without exogenous estrogen E2 (10−9 ~10−7 M). This was followed by counting the cell numbers using the trypan blue exclusion method. Data are presented as folds relative to the control and were analyzed using six independent experiments. An asterisk indicates a P value < 0.05 versus vehicle group.
