Screening to Identify Commonly Used Chinese Herbs That Affect ERBB2 and ESR1 Gene Expression Using the Human Breast Cancer MCF-7 Cell Line

Table 3

Effects of Qi-supplying and Blood-regulating herbs on ERBB2 and ESR1 gene expression.

Nature	Scientific names	Code	Dose range (μg/mL)	ERBB2 (fold of control)			ESR1 (fold of control)
				≦1.5	1.5–2	≧2	≦1.5	1.5–2	≧2
Qi-supplying	Astragalus membranaceus	A	0.3, 1, 3
	Atractylodes macrocephala	B	1, 3, 10		1*
	Poria cocos	C	0.3, 1, 3		0.3	1, 3
	Glycyrrhiza uralensis	D	0.1, 0.3, 1					0.1	0.3, 1
	Agastache rugosa	E	0.3, 1, 3		0.3, 1, 3
	Codonopsis pilosula	F	1, 3, 10						3, 10
	Zingiber officinale	G	0.1, 0.3, 1						0.1, 0.3, 1
Blood-regulating	Angelica sinensis	H	0.1, 0.3, 1		1
	Ligusticum chuanxiong	I	0.1, 0.3, 1			1			0.1, 0.3, 1
	Ziziphus jujuba	J	0.3, 1, 3
	Mucuna birdwoodiana	K	0.3, 1, 3
	Curcuma phaeocaulis	L	0.3, 1, 3		3			1, 3

ERBB2 and ESR1 gene expression was determined by luciferase reporter assay as described in the Methods section.
*Number indicates the dose that has effect with statistically significant difference (𝑃<0.05) versus vehicle group (𝑛≥6).