Screening to Identify Commonly Used Chinese Herbs That Affect ERBB2 and ESR1 Gene Expression Using the Human Breast Cancer MCF-7 Cell Line

Table 4

Effects of Heat-clearing and Yin-nourishing herbs on ERBB2 and ESR1 gene expression.

Nature	Scientific Names	Code	Dose range (μg/mL)	ERBB2 (fold of control)			ESR1 (fold of control)
				≦1.5	1.5–2	≧2	≦1.5	1.5–2	≧2
Heat-clearing	Folium nelumbinis	M	0.3, 1, 3
	Bupleurum chinense	N	1, 3, 10
	Mentha piperita	O	1, 3, 10			3*, 10
	Gardenia jasminoides	P	0.3, 1, 3						1
	Paeonia suffruticosa	Q	0.1, 0.3, 1		1
	Anemarrhena asphodeloides	R	0.03, 0.1, 0.3
	Taraxacum mongolicum	S	0.3, 1, 3
Yin-nourishing	Paeonia lactiflora	T	0.3, 1, 3
	Rehmannia glutinosa	U	0.1, 0.3, 1
	Ligustrum lucidum	V	0.3, 1, 3		0.3, 1, 3

ERBB2 and ESR1 gene expression was determined by luciferase reporter assay as described in the Methods section.
*Number indicates the dose that has effect with statistically significant difference (𝑃<0.05) versus vehicle group (𝑛≥6).