Gene Expression Profiles Underlying Selective T-Cell-Mediated Immunity Activity of a Chinese Medicine Granule on Mice Infected with Influenza Virus H1N1

Table 4

Genes associated with T-cell receptor signaling pathway.

Symbol	GenBank accession	Gene description	log2 FC^a
			M/N	SH/M	SM/M	SL/M	Oseltamivir/M
Cd3g	PH_mM_0008250	CD3 antigen, gamma polypeptide, CD3𝛾	1.26	1.43	−0.50	0.17	−0.49
Cd247	PH_mM_0012514	CD247 antigen, CD3ζ	1.56	0.38	−1.59	−0.65	−1.10
Ptprc	PH_mM_0012802	Protein tyrosine phosphatase, receptor type, C. CD45	1.65	0.32	−1.41	−0.11	−0.52
Rasgrp1	PH_mM_0009315	RAS guanyl releasing protein 1	2.43	−0.93	−1.94	−1.25	−1.60
Ctla4	mMC018651	Cytotoxic T-lymphocyte-associated protein 4	1.59	0.67	−1.94	−0.49	−1.01
Ikbkb	mMR027870	Inhibitor of kappaB kinase beta	1.33	−0.65	−0.87	−0.67	−1.19
Mapk1	mMC024552	Mitogen-activated protein kinase 1, Erk	2.23	−0.90	−1.04	−0.47	−1.50
Map3k8	mMC012006	Mitogen-activated protein kinase kinase kinase 8, COT	3.59	−2.86	−3.98	−2.92	−3.09
Nfkbie	PH_mM_0006219	Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor, epsilon. IκBε	4.26	−1.84	−3.75	−2.32	−3.13
Nfkbib	mMC008604	Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor, beta. IκBβ	3.34	−2.35	−2.68	−2.12	−2.88
Nfkbia	mMC012597	Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor, alpha. IκB𝛼	1.66	−0.92	−1.95	−1.33	−1.52
Zap70	mMC019091	Zeta-chain associated protein kinase, ZAP-70	1.53	0.38	−1.36	−0.58	−1.49
Pik3r5	PH_mM_0003125	Phosphoinositide-3-kinase, regulatory subunit 5, p101. PI3K	3.12	−2.52	−3.90	−2.95	−2.88
Pik3cg	mMC005200	Phosphoinositide-3-kinase, catalytic, gamma polypeptide. PI3K	1.64	−0.75	−1.49	−1.25	−1.21
Map2k1	PH_mM_0006215	Mitogen-activated protein kinase kinase 1	1.76	−0.53	−1.00	−0.81	−1.06
Pik3cd	PH_mM_0001034	Phosphatidylinositol 3-kinase catalytic delta polypeptide. PI3K	1.42	−0.27	−2.08	−0.74	−0.96

aFold change. The intensity ratio of probe signal criteria for differential expressions was defined as a twofold change (up or down) (log₂ FC ≥ 1 or ≤−1) in gene expression compared with virus control group and a 𝑃 value less than 0.05 in at least one time point as a minimum requirement to select. For instance, log₂ (M/N) ≥ 1 means that the comparison between normal control group and virus control group was scattered, indicating that a large number of genes in virus control group were up-regulated in response to H1N1 infection. log₂ (SH/M) ≤ −1, log₂ (SM/M) ≤ −1, log₂ (SL/M) ≤ −1, and log₂ (Oseltamivir/M) ≤ −1 means that down-regulation in gene expression attributable to the high-dose SFXF group treatment, medium-dose group SFXF treatment, low-dose SFXF group treatment and Oseltamivir group treatment was done by comparing genes in virus control group, respectively (𝑃 value data not shown).