Hexane Fractions of Bupleurum falcatum L. Stimulates Glucagon-Like Peptide-1 Secretion through -Mediated Pathway

Figure 3

Inhibition study of GLP-1 secretion using lactisole. To test the inhibitory effect of the lactisole, lactisole was treated to the NCI-H716 cells. The BF hexane fraction (HFBF) was treated with 100, 200, and 500 𝜇g/mL each to the differentiated NCI-H716 cells. GLP-1 secretion was compared with the negative control and positive control. 1% DMSO used as negative control and 2 mM Quinine (Q) used as positive control. Statistical significance was determined by a one-way ANOVA and the values are means ± SEM; ^∗𝑃<0.05 and ^∗∗∗𝑃<0.0001 versus 1% DMSO. ^#𝑃<0.05 and ^##𝑃<0.001 lactisole and HFBF treated group versus only HFBF treated group.