Hexane Fractions of Bupleurum falcatum L. Stimulates Glucagon-Like Peptide-1 Secretion through -Mediated Pathway

Figure 4

HFBF stimulates GLP-1 secretion through G_𝛽𝛾 pathway. Treatment of BF hexane fraction at 100 seconds into the medium increased the concentration of Ca²⁺ in cytosol. With BF hexane fraction, inhibitors were treated. (a) Gallein which is an inhibitor of G_𝛽𝛾 subunit-dependent signaling pathway reduced GLP-1 secretion dose dependently. (b) 2APB inhibits the IP₃-induced Ca²⁺ release. 2APB also reduced GLP-1 secretion dose dependently. (c) U73122 is an inhibitor of PLC-dependent processes. With this inhibitor, concentration of Ca²⁺ showed less change despite treatment of BF hexane fraction. Data are means of intensity of the NCI-H716 cells (𝑛=10). ^∗∗∗𝑃<0.0001 versus BF hexane fraction 100 𝜇g/mL.

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