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Evidence-Based Complementary and Alternative Medicine
Volume 2014 (2014), Article ID 982913, 11 pages
http://dx.doi.org/10.1155/2014/982913
Research Article

Potential Mechanisms of an Antiadenomyosis Chinese Herbal Formula Shaoyao-Gancao Decoction in Primary Cell Culture Model

1First Affiliated Hospital, Guangzhou University of Chinese Medicine, Guangzhou 510405, China
2School of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou 510006, China
3Guangdong Second Province Hospital of Traditional Chinese Medicine, Guangzhou 510095, China
4School of Nursing, Guangzhou University of Chinese Medicine, Guangzhou 510405, China

Received 5 August 2014; Revised 11 October 2014; Accepted 15 October 2014; Published 10 November 2014

Academic Editor: Min Ye

Copyright © 2014 Yong-Ge Guan et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background. Shaoyao-Gancao Decoction (SGD), a well-known traditional Chinese medicine prescription, has been widely used to treat adenomyosis, dysmenorrhea, abdominal pain, and inflammation in Asia. However, the mechanism underlying the effectiveness of SGD in the treatment of adenomyosis still remains elusive. The present study aimed to investigate the bioactivity of SGD and its underlying molecular mechanisms using cultured human adenomyosis-derived cells. Methods. Human adenomyosis-derived cells were treated with SGD and its major constituents (paeoniflorin and liquiritin) in vitro. Effects of SGD, paeoniflorin, and liquiritin on cell proliferation and apoptosis were examined by MTT assay and flow cytometry analyses. The effects of SGD, paeoniflorin, and liquiritin on the production of PGE2 and PGF2α were assayed using ELISA. ER-α and OTR mRNA expression levels were also evaluated by real-time qRT-PCR. Results. SGD, paeoniflorin, and liquiritin inhibited proliferation and induced apoptosis of human adenomyosis-derived cells in a dose-dependent manner. SGD and paeoniflorin significantly reduced the PGE2 and PGF2α production. Furthermore, they remarkably decreased the mRNA levels of ER-α and OTR. Conclusions. The results of this study provide possible mechanisms for the bioactivity of SGD for treating adenomyosis and contribute to the ethnopharmacological knowledge about this prescription.