Antiatherosclerotic Effect of Korean Red Ginseng Extract Involves Regulator of G-Protein Signaling 5

Figure 4

The effect of KRGE on proinflammatory mediators in LPS-activated RAW264.7 cells. RAW264.7 cells were preincubated with KRGE for 30 min and then stimulated with LPS (0.1 μg/mL) for 18 h. The cell supernatant was transferred to 96 wells and mixed with Griess reagent and NO release was measured, as described in Section 2 (a). The effect of KRGE on cell viability was measured by MTT assay (b). The total RNA was isolated and mRNA expression levels were determined by RT-PCR for iNOS, COX-2, IL-1β (c), and TNF-α and IL-6 (d). GAPDH was used as a loading control. Images are represented of 4 different experiments. Values in bar graphs are mean ± SEM of 4 independent experiments. , , versus LPS control.

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