Purple Sweet Potato Leaf Extract Induces Apoptosis and Reduces Inflammatory Adipokine Expression in 3T3-L1 Differentiated Adipocytes

Figure 1

Separation of adipocytes and preadipocytes from differentiated 3T3-L1 cells by Percoll density gradient centrifugation. 3T3-L1 preadipocytes were grown to confluence and then in the absence (A) or presence (B) of adipogenic agents, which induced cellular differentiation. The differentiated 3T3-L1 cells, which included both adipocytes (arrow) and preadipocytes, were separated by Percoll gradient centrifugation (a). After centrifugation, adipocytes (A) and preadipocytes (B) were cultured on collagen-coated dishes overnight and then stained with Oil Red O (b). The scale bar equals 30 μm.

(a)

(b)