Purple Sweet Potato Leaf Extract Induces Apoptosis and Reduces Inflammatory Adipokine Expression in 3T3-L1 Differentiated Adipocytes

Figure 3

Determination of PSPLE-induced apoptosis of 3T3-L1 adipocytes. The differentiated 3T3-L1 cells were cultured with PSPLE at 0 (A), 1 (B), 2 (C), and 4 (D) mg/mL for 72 h. Then, the cells were stained with DAPI and were observed under a fluorescence microscope (a). In addition, the cells were incubated with FITC-conjugated annexin-V and propidium iodide (PI) and were measured by flow cytometry (b). Normal cells were annexin-V-negative and PI-negative (Q3); cells in early apoptosis were annexin-V-positive and PI-negative (Q4); cells in late apoptosis/necrosis were annexin-V-positive and PI-positive (Q2). The scale bar equals 30 μm.

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(b)